Fluorogenic Substrates for Assay of Chymosin

V. V. Starovoitova^*, I. Yu. Filippova, E. N. Lysogorskaya, E. S. Oksenoit, and G. I. Lavrenova

Department of Natural Compounds Chemistry, School of Chemistry, Lomonosov Moscow State University, Moscow, 119899 Russia; fax: (095)932-8846; E-mail: violst@genebee.msu.su

^* To whom correspondence should be addressed.

Received September 1, 1999; Revision received October 1, 1999

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The use of fluorogenic substrates with intramolecular fluorescence quenching as substrates for chymosin was studied. It was shown that chymosin hydrolyzes the Phe-Phe peptide bond. The effect of pH on the hydrolysis of substrates by chymosin was investigated. The catalytic characteristics of the hydrolysis of the fluorogenic substrates were obtained at the pH optima. The influence of dimethylformamide on chymosin activity was studied.

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KEY WORDS:chymosin, activity determination, fluorogenic substrates

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