Adenosine Transport in Liver Before and After Organ Preservation

V. Yu. Toshchakov^*, L. V. Bashkina, G. A. Vityazev, and V. I. Shumakov

Institute for Transplantation and Artificial Organs, Ministry of Public Health of the Russian Federation, Shchukinskaya ul. 1, Moscow, 123182 Russia; fax: (095) 943-0008; E-mail: vtoeto@sonnet.ru

^* To whom correspondence should be addressed.

Received August 17, 1998; Revision received October 29, 1998

---

The effect of 24-h cold storage of liver on nucleoside transport was investigated. Nucleoside transport was estimated under conditions when both known types of nucleoside transport, facilitated diffusion and Na⁺/nucleoside cotransport, were active and when one of these transport mechanisms was inhibited. The rate of adenosine transport was not decreased after long-term cold storage of the liver. Inhibition of one of the transport systems decreased the rate of adenosine uptake before and after preservation of the liver to about the same extent. The adenosine transport rate was maintained during long-term (100 min) liver perfusion ex vivo. Slight activation of energy-dependent transport in the beginning of reperfusion and the slower recovery of this transport after the second transition from Na⁺-free to Na⁺-containing perfusion are not regarded as physiologically important because they were observed after changing the ionic content of the extracellular medium. We conclude that the nucleoside transport systems in liver are quite well preserved after 24-h cold storage of the organ.

---

KEY WORDS: nucleoside transport, liver preservation, adenosine, dipyridamole, Na⁺-free perfusion

---