Extracellular Oxidases from Solid-State Culture of the Ligninolytic Fungus Panus tigrinus 8/18

N. N. Pozdnyakova, A. A. Leontievsky^*, and L. A. Golovleva

Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia; fax: (095) 923-3602; E-mail: leont@ibpm.serpukhov.su

^* To whom correspondence should be addressed.

Received July 1, 1998; Revision received September 12, 1998

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Three extracellular oxidases were purified and characterized from a solid-state culture of the ligninolytic fungus Panus tigrinus 8/18. Oxidases 1 and 2 have physicochemical properties and substrate specificity typical for laccases but have no "blue" maximum in the absorption spectra. They seem to be forms of modified "yellow" laccase. The absorption spectrum of oxidase 4 is similar to that of oxidases 1 and 2. However, the molecular weight (35 kD) and substrate specificity (no reaction with guaiacol, catechol, syringic acid, and syringaldazine) are different.

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KEY WORDS: laccase, white rot fungi, substrate specificity

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