Biosynthesis of Chlorophyll P-680 of Photosystem II in Greening Leaves of Plants Adapted to Heat Shock

N. V. Ignatov^*, S. A. Gostimskii, L. Ya. Satina, and F. F. Litvin

Department of Physico-Chemical Biology, School of Biology, Lomonosov Moscow State University, Moscow, 119899 Russia; E-mail: ozrina@jnlbioch.bio.msu.su

^* To whom correspondence should be addressed.

Received February 12, 1998

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In etiolated pea and maize leaves illuminated after incubation at 38°C, a new dark reaction was shown manifested in the bathochromic shift of spectral bands and accompanied by esterification of the product of protochlorophyllide photochemical reduction--Chld 684/676: Chld 684/676 --> Chl 688/680. After completion of the reaction a rapid (20-30 sec) quenching of the fluorescence of the reaction product (Chl 688/680) was observed. The reaction Chld 684/676 --> Chl 688/680 is inhibited under anaerobic conditions and in the presence of cyanide; the reaction accompanied by Chl 688/680 fluorescence quenching is not observed in pea mutants with impaired function of photosystem II reaction centers. The spectral properties of the formed Chl form with the absorption maximum at 680 nm, fluorescence quenching, and simultaneous synthesis of pheophytin suggest that the reaction is connected with the chlorophyll of photosystem II reaction center--P-680.

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KEY WORDS: chlorophyll a biosynthesis, protochlorophyllide, reaction centers of photosystem II, heat shock, etiolated leaves

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