Spectrophotometric Quantitative Analysis of the Main Hemoglobin Derivatives

S. A. Adamov, S. A. Aleksandrova, A. N. Denisov, E. Yu. Mosur, and N. A. Semikolenova^*

Sensor Microelectronics Institute, Siberian Branch of the Russian Academy of Sciences, pr. Mira 55a, Omsk, 644077 Russia; fax: (3812) 64-8676; E-mail: semikole@univer.omsk.su

^* To whom correspondence should be addressed.

Received October 20, 1997

---

A spectrophotometric multiwavelength method for the simultaneous determination of the main hemoglobin derivatives including oxyhemoglobin, deoxyhemoglobin, carboxyhemoglobin, and methemoglobin has been developed. The choice of analytical wavelengths was made by the methods of information factors and linear programming. The software for the quantitative analysis was developed considering the least square method, linear programming, algebraic background correction, and combined methods including linear programming and algebraic background correction.

---

KEY WORDS: hemoglobin, software, spectrophotometry

---