Site-Specific Endonuclease SscL1 I from Strain Staphylococcus Species L1

L. Yu. Vasiljeva¹, L. A. Zheleznaya², and N. I. Matvienko^1*

¹Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia; fax: (095) 924-0493; E-mail: protres@sovam.com

²Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia; fax: (095) 135-6219.

^* To whom correspondence should be addressed.

Received August 12, 1997; Revision received October 8, 1997

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A site-specific endonuclease SscL1 I preparation has been isolated and purified to near homogeneity from the strain Staphylococcus sp. L1 without admixtures of other nuclease activity. DNA cleavage proceeds according to the scheme:

5´-G^ANTC-3´
3´-CTNA^G-5´,

and thus the isolated enzyme is an isoschizomer of restriction endonuclease HinfI and belongs to the second class of restriction endonucleases. SscL1 I works over a broad range of temperature and pH. The enzyme is characterized by high stability during storage.

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KEY WORDS: site-specific endonuclease, isoschizomer, HinfI

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