Structure of a Neutral O-Specific Polysaccharide of the Bacterium Proteus mirabilis O24
S. N. Senchenkova,1 G. V. Zatonskii,1 E. Ujazda,2 A. S. Shashkov,1 W. Kaca,2 and Yu. A. Knirel1,3
1Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii pr. 47, Moscow, 117913 Russia; fax: (095) 135-5328; E-mail: knirel@ioc.ac.ru2Center for Microbiology and Virology, Polish Academy of Sciences, ul. Lodowa 106, 93-232 Lodz, Poland; fax: (48-42) 49-1633.
3To whom correspondence should be addressed.
Submitted July 22, 1997.
Lipopolysaccharide of the bacterium Proteus mirabilis O24 was found to have a neutral O-specific polysaccharide chain containing D-galactose, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-galactose in ratios 1:2:1. On the basis of 1H- and 13C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected 1H,13C heteronuclear multiple-quantum coherence (HMQC), and nuclear Overhauser effect spectroscopy (NOESY), the following structure of the branched tetrasaccharide repeating unit of the O-specific polysaccharide was established:
KEY WORDS: lipopolysaccharide, O-antigen, bacterial polysaccharide, structure, NMR spectroscopy, Proteus mirabilis.
