Modification of Catalase by Chondroitin Sulfate

A. V. Maksimenko^1,2 and E. G. Tischenko¹

¹Institute of Experimental Cardiology, Russian Cardiology Research Center, ul. 3-ya Cherepkovskaya 15a, Moscow, 121552 Russia; fax: (095) 415-2962; E-mail: csc@adonis.ias.msk.su

²To whom correspondence should be addressed.

Submitted July 2, 1997; revision submitted July 16, 1997.

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Catalase was chemically modified by sodium chondroitin sulfate using the benzoquinone binding method. Thus, 40-42% of the catalase preparation was modified. Treatment of catalase and superoxide dismutase with benzoquinone-activated chondroitin sulfate results in a bienzymic conjugate with electrophoretically heterogenous composition. The yield of the products and their residual catalytic activity indicate that the method can be used for the preparation of modified catalase and the bienzymic conjugate to study their efficiency in vivo.

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KEY WORDS: catalase, chondroitin sulfate, superoxide dismutase, chemical modification, covalent conjugation.

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