Site-Specific DNA-Methylase M·BspST5I Methylates Only One Strand of the Recognized Site

N. N. Matvienko,¹ L. A. Zheleznaya,² N. V. Zelinskaya,³ and N. I. Matvienko^3,4

¹Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia.

²Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia; fax: (7-095) 135-62-19.

³Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia; fax: (7-095) 924-04-93; E-mail: protres@sovam.com

⁴To whom correspondence should be addressed.

Submitted October 24, 1996; revision submitted December 16, 1996.

We recently isolated a site-specific adenine DNA-methylase, M·BspST5I, which methylates only one strand of the recognized site GC***ATC. The methylated base is indicated by an asterisk.

KEY WORDS: site-specific DNA-methylase, restriction-modification, S-adenosyl methionine.

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