Development of Immunoenzymatic Reactions of Peptides Using Recombinant Hybrid Proteins

I. K. Mandrika,^1,2 V. R. Baumanis,¹ I. V. Jansone,¹ A. Ya. Skangals,¹ and V. M. Berzins¹

¹Center of Biomedical Research, University of Latvia, Ratsupites 1, Riga, LV1067 Latvia; fax: (3712) 42-75-21.

²To whom correspondence should be addressed.

Submitted September 23, 1996; revision submitted November 18, 1996.

A recombinant hybrid protein comprising the bacteriophage fr coat protein, a short linker, and atrial natriuretic factor (ANF) as well as native and recombinant phage coat proteins, ANF, and variants of the hybrid recombinant protein were used in the development of various immunological reactions including immunization, preparation of affinity columns, purification of antibodies, synthesis of conjugates, and immunoenzyme assay of ANF and the recombinant protein. The hybrid protein is effective in competitive assay of ANF and other constructs that include the phage protein.

KEY WORDS: recombinant proteins, immunoanalysis, natriuretic factor.

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