Steady-State Kinetics of NADH Oxidation by Hydrogen Peroxide in the Presence of Horseradish Peroxidase

O. V. Lebedeva^1,2 and N. N. Ugarova¹

¹School of Chemistry, Lomonosov Moscow State University, Moscow, 119899 Russia; fax: (7-095) 939-35-89.

²To whom correspondence should be addressed.

Submitted October 14, 1996.

The steady-state kinetics of NADH peroxidation was studied. Conditions were determined under which the oxidase pathway of NADH oxidation does not take place. The values of k_cat and K_m were determined at pH 4.7-8.2. Nucleophilic compounds do not activate the NADH peroxidation; the mechanisms of oxidation of NADH and inorganic ions are similar.

KEY WORDS: horseradish peroxidase, peroxidation, NADH.

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