Automatic Procedures on Porous Membranes: Counterflow Isotachophoresis and Immuno-Electrochromatography

G. I. Abelev^1,2 and E. R. Karamova¹

¹Institute of Carcinogenesis, Cancer Research Center, Russian Academy of Medical Sciences, Kashirskoe shosse 24, Moscow, 115478 Russia; fax: (7-095) 324-12-05.

²To whom correspondence should be addressed.

Submitted June 11, 1996.

Two related methods developed by the authors are reviewed - counterflow isotachophoresis and immuno-electrochromatography on porous membranes. The characteristics of electro-osmosis in porous membranes during isotachophoresis are important for both methods. Principles and applications of the methods are described including electrophoretic analysis of low-protein biological liquids and automatic multistep immunochemical reactions such as Western blotting, epitope analysis of monoclonal antibodies, and multiantigen tests. The methods can be used for the detection of DNA-binding proteins.

KEY WORDS: isotachophoresis, electroendosmosis, counterflow isotachophoresis, automatic immunoblotting, acetate cellulose membrane, immuno-electrochromatography, epitope analysis, DNA-binding protein.

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